Cavity-Enhanced Raman Spectroscopy in the Biosciences: In Situ, Multicomponent, and Isotope Selective Gas Measurements To Study Hydrogen Production and Consumption by Escherichia coli.

Cavity-Enhanced Raman Spectroscopy in the Biosciences: In Situ, Multicomponent, and Isotope Selective Gas Measurements To Study Hydrogen Production and Consumption by Escherichia coli.

Our recently introduced a cavity-enhanced Raman spectroscopy (CER) with optical feedback cw diode laser as a sensitive analytical tool. Here we report the improvements made in the techniques and applications in the biosciences’s first in situ, multicomponent, and isotope selective gas measurements for hydrogen production and consumption studies by Escherichia coli.

Under anaerobic conditions, cultures grown in rich media comes with D-glucose or glycerol produce H2 and simultaneously consumes most. By introducing D2 in the upper chamber, hydrogen production and consumption can be separated because of the different spectroscopic signature of isotopomers. the different phases with clearly different kinetic regimes of H2 and CO2 production and consumption D2 identified. Some D2 consumed is converted back to H2 through H / D exchange with the solvent. HD formed only as a minor component.

This reflects both that the H / D exchange on hydrogenase active site that is faster than the rate of recombination, fast HD reclaim happens after the molecule is formed, or that the site where oxidation and reduction D2 proton occurs are physically separated. While the culture of glucose plus, the addition of D2 causes an increase in H2 generated, while the results of the CO2 remains unchanged; with glycerol, the addition of D2 lead not only to increase the yield of H2, but also significantly increases the production of CO2, reflecting the impact of the fermentation pathway. The addition of CO was found to completely inhibit the production of H2 and significantly reduce the oxidation of D2, showed at least some role for O2-tolerant Hyd-1 consumption D2.


effective involvement of nursing students in the biosciences studies remains a challenge for many tertiary institutions. In this study we sought to apply and then evaluate simple hand-intervention, which consisted of a series of hand-games and puzzles, to improve nursing student engagement with learning core concepts and anatomy involved in clinical anatomy and physiology.

This study uses longitudinal Quazi-experimental before and after design, to explore the effects of interventions on the performance of student learning. Set in three different campuses of the same university, was included in 1320 first-year nursing students from 2013 to 2014 who were studying Anatomy and Physiology. Students are exposed to the intervention or not, and the concurrent performance of academic, weekly quiz score, performance in two weeks worksheets and, in the semester, the performance comparison test.

 Cavity-Enhanced Raman Spectroscopy in the Biosciences: In Situ, Multicomponent, and Isotope Selective Gas Measurements To Study Hydrogen Production and Consumption by Escherichia coli.
Cavity-Enhanced Raman Spectroscopy in the Biosciences: In Situ, Multicomponent, and Isotope Selective Gas Measurements To Study Hydrogen Production and Consumption by Escherichia coli.

Improving Education in Agricultural Biosciences through Study Abroad in the United States.

Studying abroad in agricultural biosciences to develop cultural, academic and communication skills of students and improve the work. However, in the United States, study abroad discussion is limited to one direction directionality (US students to other countries) or experience of international students coming to the US for a program title. We analyze the perspectives and experiences of studying abroad by Zamorano University (Honduras) students who completed pasantía Bioscience agriculture (four-month) during the last year of their degree program.

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We use a mix of methods to collect data through focus groups and surveys with Zamorano students who have completed pasantía in 2017, as well as key informant interviews with coordinators pasantía Zamorano. experience studying abroad than among students who complete their pasantía in the United States (37%) and those who completed their pasantía in each of the 17 other countries around the world.

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