Relative Performance of MinION (Oxford Nanopore Technologies) vs. Sequel (Pacific Biosciences) Third-Generation Sequencing Instruments in Identification of Agricultural and Forest Fungal Pathogens.

Relative Performance of MinION (Oxford Nanopore Technologies) vs. Sequel (Pacific Biosciences) Third-Generation Sequencing Instruments in Identification of Agricultural and Forest Fungal Pathogens.

molecular identification method based on culture-has revolutionized the detection of pathogens, however, this method is slow and may produce inconclusive results of environmental materials. The second-generation sequencing tools have further enhanced the precision and sensitivity of detection, but this analysis is expensive and may take several days to months. The third-generation sequencing techniques, the device is portable Minion (Oxford Nanopore Technologies) has received much attention because of its size and the possibility of a quick analysis of small low cost.

Here, we compare the relative performance of the two instruments third generation sequencing, Minion and Sequel (Pacific Biosciences) in the identification and diagnosis of fungi and oomycetes pathogens of conifers (Pinaceae spp.) A needle and a potato (Solanum tuberosum) leaves and tubers. We show that the sequel efficient instrument for metabarcoding complex samples, while Minion is not suitable for this purpose because of the high error rate and some bias. However, we discovered that the minion can be used for rapid and accurate identification of the dominant pathogenic organisms and other related organisms from plant tissue follows both based on PCR amplicon-free-and metagenomics approach.

Using metagenomics approach to the extraction of DNA and shortened incubation times, we did the whole workflow from sample preparation through Minion DNA extraction, sequencing, bioinformatics and interpretation in two and a half hours. We recommend the use of Minion for rapid diagnosis of pathogens and other potential organisms, but care needs to be taken to control or account for some of the technical potential biases.IMPORTANCE Microbial pathogens causing huge losses to agriculture and forestry, but culturing- combined current and molecular-based detection method identification-too slow for the rapid identification and implementation of countermeasures.

Here we develop new protocols and quick for third-generation diagnostic Minion-based Oxford Nanopore plant pathogens greatly improve diagnostic speed. However, due to a high error rate and the technical bias in Minion, Pacific BioSciences Sequel platform more useful for deep amplicon based biodiversity monitoring (metabarcoding) from complex environmental samples.

 Relative Performance of MinION (Oxford Nanopore Technologies) vs. Sequel (Pacific Biosciences) Third-Generation Sequencing Instruments in Identification of Agricultural and Forest Fungal Pathogens.
Relative Performance of MinION (Oxford Nanopore Technologies) vs. Sequel (Pacific Biosciences) Third-Generation Sequencing Instruments in Identification of Agricultural and Forest Fungal Pathogens.

Integrative Biology and Big-Data-centrism: Mapping a Bioscience Ethics Perspective with S.W.O.T. Matrix.

In biomedicine today, omics technology-oriented system encourages research mode to achieve a more holistic view and personal health and disease. This scientific approach shifts co-occur with biocapitalism era marked by the market for biomaterials (eg, DNA, cells and tissues) as resource exploitation, high-throughput technology as a tool, and “Big Data” as currency.

Prediagnostics and based genomic analysis successfully entered the public domain more or less unfiltered, offers a variety of business opportunities imagine individuals to contribute to the health sector by providing biomaterials and data, and using technology, thus becoming participants and coproducers health information. Explore the strengths and weaknesses, opportunities and threats by S.W.O.T. analysis, we highlight some of the opportunities, pitfalls, and the sector bias of ethical attitudes Bioscience.

Explore the strengths and weaknesses, opportunities and threats by S.W.O.T. analysis, we highlight some of the opportunities, pitfalls, and the sector bias of ethical attitudes Bioscience. We conclude that the shift from predictive diagnostic interpretation of data that comes along with integrative biology seems to escape occasional awareness of the public and experts.

Mouse Histone Deacetylase 2 (HDAC2) ELISA Kit

DLR-HDAC2-Mu-48T 48T
EUR 527
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Histone Deacetylase 2 (HDAC2) in samples from tissue homogenates, cell lysates or other biological fluids.

Mouse Histone Deacetylase 2 (HDAC2) ELISA Kit

DLR-HDAC2-Mu-96T 96T
EUR 688
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Histone Deacetylase 2 (HDAC2) in samples from tissue homogenates, cell lysates or other biological fluids.

Human Histone Deacetylase 2 (HDAC2) ELISA Kit

RDR-HDAC2-Hu-48Tests 48 Tests
EUR 544

Human Histone Deacetylase 2 (HDAC2) ELISA Kit

RDR-HDAC2-Hu-96Tests 96 Tests
EUR 756

Mouse Histone Deacetylase 2 (HDAC2) ELISA Kit

RDR-HDAC2-Mu-48Tests 48 Tests
EUR 557

Mouse Histone Deacetylase 2 (HDAC2) ELISA Kit

RDR-HDAC2-Mu-96Tests 96 Tests
EUR 774

Human Histone Deacetylase 2 (HDAC2) ELISA Kit

RD-HDAC2-Hu-48Tests 48 Tests
EUR 521

Human Histone Deacetylase 2 (HDAC2) ELISA Kit

RD-HDAC2-Hu-96Tests 96 Tests
EUR 723

Mouse Histone Deacetylase 2 (HDAC2) ELISA Kit

RD-HDAC2-Mu-48Tests 48 Tests
EUR 533

Mouse Histone Deacetylase 2 (HDAC2) ELISA Kit

RD-HDAC2-Mu-96Tests 96 Tests
EUR 740

Immunoprecipitation Kit (OKRA00051)

OKRA00051 1 kit
EUR 1040
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Topoisomerase II Immunoprecipitation Kit

TG1035 >50 analyses
EUR 548

Immunoprecipitation Kit: Protein A-Agarose

BS688 20Rxn, 20prep
EUR 202.25

Immunoprecipitation Kit: Protein A-Sepharose

BS690 20Rxn, 20prep
EUR 197.9

EpiQuik Chromatin Immunoprecipitation (ChIP) Kit

P-2002 96 Reactions
EUR 904.05
Description: Ask the seller for details

EpiQuik Methylated DNA Immunoprecipitation Kit

P-2019 48 Reactions
EUR 889.55
Description: Ask the seller for details

HDAC2 Protein

E24005 50 µg
EUR 806.9
Description: Ask the seller for details

HDAC2 siRNA

20-abx919177
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

HDAC2 siRNA

20-abx919178
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

HDAC2 Antibody

32590-100ul 100ul
EUR 252

HDAC2 Antibody

1-CSB-PA260045
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against HDAC2. Recognizes HDAC2 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:5000, IHC:1:25-1:100

HDAC2 Antibody

1-CSB-PA010238GA01HU
  • EUR 597.00
  • EUR 333.00
  • 150ul
  • 50ul
Description: A polyclonal antibody against HDAC2. Recognizes HDAC2 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF

HDAC2 Antibody

1-CSB-PA017043
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against HDAC2. Recognizes HDAC2 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:2000, IHC:1:15-1:50

HDAC2 Antibody

1-CSB-PA002879
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against HDAC2. Recognizes HDAC2 from Human, Mouse, Rat, Monkey. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/40000

HDAC2 Antibody

48802-100ul 100ul
EUR 333

HDAC2 Antibody

48802-50ul 50ul
EUR 239

HDAC2 Antibody

49162-100ul 100ul
EUR 333

HDAC2 Antibody

49162-50ul 50ul
EUR 239

HDAC2 Antibody

AF6470 200ul
EUR 304
Description: HDAC2 Antibody detects endogenous levels of total HDAC2.

HDAC2 Antibody

DF6808 200ul
EUR 304
Description: HDAC2 Antibody detects endogenous levels of total HDAC2.

HDAC2 Antibody

ABD6808 100 ug
EUR 438

HDAC2 Antibody

ABF6470 100 ug
EUR 438

HDAC2 Antibody

3602-100
EUR 316

HDAC2 Antibody

3602-30T
EUR 146

anti-HDAC2

YF-PA12274 100 ug
EUR 403
Description: Rabbit polyclonal to HDAC2

HDAC2 antibody

PAab09976 100 ug
EUR 386

HDAC2 antibody

20R-2037 50 ug
EUR 281
Description: Rabbit polyclonal HDAC2 antibody

HDAC2 antibody

20R-2315 50 ug
EUR 281
Description: Rabbit polyclonal HDAC2 antibody

HDAC2 antibody

70R-17705 50 ul
EUR 435
Description: Rabbit polyclonal HDAC2 antibody

HDAC2 antibody

70R-37578 100 ug
EUR 273
Description: Rabbit Polyclonal HDAC2 antibody

HDAC2 antibody

70R-11942 100 ug
EUR 403
Description: Rabbit polyclonal HDAC2 antibody

HDAC2 antibody

70R-13892 100 ug
EUR 322
Description: Affinity purified Rabbit polyclonal HDAC2 antibody

HDAC2 antibody

70R-13893 100 ug
EUR 322
Description: Affinity purified Rabbit polyclonal HDAC2 antibody

HDAC2 antibody

70R-34167 100 ug
EUR 327
Description: Rabbit polyclonal HDAC2 antibody

HDAC2 Peptide

7899P 0.05 mg
EUR 164.75
Description: (CT) HDAC2 Peptide

HDAC2 Antibody

7899-002mg 0.02 mg
EUR 171.82
Description: The histone deacetylase (HDAC) family contains multiple members which are divided into four classes. Class I of the HDAC family comprises four members, HDAC1, 2, 3, and 8, each of which contains a deacetylase domain and exhibits a different, individual substrate specificity and function in vivo (1). HDAC2 was first identified as as a mammalian homolog to the yeast transcriptional regulator RPD3 (2). HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor (2,3). Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events (4).

HDAC2 Antibody

7899-01mg 0.1 mg
EUR 436.42
Description: The histone deacetylase (HDAC) family contains multiple members which are divided into four classes. Class I of the HDAC family comprises four members, HDAC1, 2, 3, and 8, each of which contains a deacetylase domain and exhibits a different, individual substrate specificity and function in vivo (1). HDAC2 was first identified as as a mammalian homolog to the yeast transcriptional regulator RPD3 (2). HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor (2,3). Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events (4).

Coelenterazine ip

10116 50uG
EUR 114
Description: Minimum order quantity: 1 unit of 50uG

Coelenterazine ip

10116-1 1MG
EUR 725
Description: Minimum order quantity: 1 unit of 1MG

Coelenterazine ip

10116-2 250uG
EUR 259
Description: Minimum order quantity: 1 unit of 250uG

EpiQuik Hydroxymethylated DNA Immunoprecipitation (hMeDIP) Kit

P-1038 96 Reactions
EUR 1672.55
Description: reagents widely cited

EpiQuik Tissue Chromatin Immunoprecipitation (ChIP) Kit

P-2003 96 Reactions
EUR 904.05
Description: reagents widely cited

EpiQuik Tissue Methylated DNA Immunoprecipitation Kit

P-2020 48 Reactions
EUR 889.55
Description: The best epigenetics products

IP-10/ Rat IP- 10 ELISA Kit

ELA-E0371r 96 Tests
EUR 886

Anti-HDAC2 Antibody

PA1350 100ug/vial
EUR 294

Anti-HDAC2 Antibody

PA1350-2 100ug/vial
EUR 334

HDAC2 Polyclonal Antibody

ES2499-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against HDAC2 from Human/Mouse/Rat/Monkey. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

HDAC2 Polyclonal Antibody

ES2499-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against HDAC2 from Human/Mouse/Rat/Monkey. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

HDAC2 Monoclonal Antibody

A-4002 100 µg Ask for price
Description: Ask the seller for details

HDAC2 Polyclonal Antibody

A-4709 100 µl
EUR 677.85
Description: fast delivery possible

HDAC2 Rabbit pAb

A0867-100ul 100 ul
EUR 308

HDAC2 Rabbit pAb

A0867-200ul 200 ul
EUR 459

HDAC2 Rabbit pAb

A0867-20ul 20 ul
EUR 183

HDAC2 Rabbit pAb

A0867-50ul 50 ul
EUR 223

HDAC2 (pS394) Antibody

abx010896-100ug 100 ug
EUR 439

HDAC2 (pS394) Antibody

20-abx000253
  • EUR 495.00
  • EUR 704.00
  • EUR 356.00
  • 100 ul
  • 200 ul
  • 50 ul

HDAC2 Polyclonal Antibody

ABP51500-003ml 0.03ml
EUR 158
Description: A polyclonal antibody for detection of HDAC2 from Human, Mouse, Rat, Monkey. This HDAC2 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human HDAC2 around the non-phosphorylation site of S394

HDAC2 Polyclonal Antibody

ABP51500-01ml 0.1ml
EUR 289
Description: A polyclonal antibody for detection of HDAC2 from Human, Mouse, Rat, Monkey. This HDAC2 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human HDAC2 around the non-phosphorylation site of S394

HDAC2 Polyclonal Antibody

ABP51500-02ml 0.2ml
EUR 414
Description: A polyclonal antibody for detection of HDAC2 from Human, Mouse, Rat, Monkey. This HDAC2 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human HDAC2 around the non-phosphorylation site of S394

HDAC2 Rabbit pAb

A13618-100ul 100 ul
EUR 308

HDAC2 Rabbit pAb

A13618-200ul 200 ul
EUR 459

HDAC2 Rabbit pAb

A13618-20ul 20 ul
EUR 183

HDAC2 Rabbit pAb

A13618-50ul 50 ul
EUR 223

HDAC2 Rabbit mAb

A19626-100ul 100 ul
EUR 410

HDAC2 Rabbit mAb

A19626-200ul 200 ul
EUR 571

HDAC2 Rabbit mAb

A19626-20ul 20 ul
EUR 221

HDAC2 Rabbit mAb

A19626-50ul 50 ul
EUR 287

HDAC2 Rabbit pAb

A2084-100ul 100 ul
EUR 308

HDAC2 Rabbit pAb

A2084-200ul 200 ul
EUR 459

HDAC2 Rabbit pAb

A2084-20ul 20 ul
EUR 183

HDAC2 Rabbit pAb

A2084-50ul 50 ul
EUR 223

HDAC2-specific Antibody

abx233797-100ug 100 ug
EUR 481

Anti-HDAC2 antibody

STJ111039 100 µl
EUR 277
Description: This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants.

Anti-HDAC2 antibody

STJ93478 200 µl
EUR 197
Description: Rabbit polyclonal to HDAC2.

Polyclonal HDAC2 Antibody

APR06887G 0.1 mg
EUR 659
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human HDAC2 . This antibody is tested and proven to work in the following applications:

Anti-HDAC2 antibody

STJ23929 100 µl
EUR 277
Description: This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants.

HDAC2 Polyclonal Antibody

40995-100ul 100ul
EUR 252

HDAC2 Polyclonal Antibody

40995-50ul 50ul
EUR 187

anti- HDAC2 antibody

FNab03796 100µg
EUR 505.25
Description: Antibody raised against HDAC2

HDAC2 Conjugated Antibody

C48802 100ul
EUR 397

Polyclonal HDAC2 Antibody

APR00146G 0.1mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human HDAC2 . This antibody is tested and proven to work in the following applications:

Anti-HDAC2 antibody

STJ115577 100 µl
EUR 277
Description: This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants.

Recombinant Human HDAC2

P0310 100ug
EUR 522.36
Description: Recombinant Human protein for HDAC2

anti- HDAC2 antibody

FNab09976 100µg
EUR 548.75
Description: Antibody raised against HDAC2

In addition, the rapid translation into products for the global healthcare market is based on the valuable views on health and disease, which in turn affects the basic research through, for example, funding policy and research questions that are asked.

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