Relative Performance of MinION (Oxford Nanopore Technologies) vs. Sequel (Pacific Biosciences) Third-Generation Sequencing Instruments in Identification of Agricultural and Forest Fungal Pathogens.
molecular identification method based on culture-has revolutionized the detection of pathogens, however, this method is slow and may produce inconclusive results of environmental materials. The second-generation sequencing tools have further enhanced the precision and sensitivity of detection, but this analysis is expensive and may take several days to months. The third-generation sequencing techniques, the device is portable Minion (Oxford Nanopore Technologies) has received much attention because of its size and the possibility of a quick analysis of small low cost.
Here, we compare the relative performance of the two instruments third generation sequencing, Minion and Sequel (Pacific Biosciences) in the identification and diagnosis of fungi and oomycetes pathogens of conifers (Pinaceae spp.) A needle and a potato (Solanum tuberosum) leaves and tubers. We show that the sequel efficient instrument for metabarcoding complex samples, while Minion is not suitable for this purpose because of the high error rate and some bias. However, we discovered that the minion can be used for rapid and accurate identification of the dominant pathogenic organisms and other related organisms from plant tissue follows both based on PCR amplicon-free-and metagenomics approach.
Using metagenomics approach to the extraction of DNA and shortened incubation times, we did the whole workflow from sample preparation through Minion DNA extraction, sequencing, bioinformatics and interpretation in two and a half hours. We recommend the use of Minion for rapid diagnosis of pathogens and other potential organisms, but care needs to be taken to control or account for some of the technical potential biases.IMPORTANCE Microbial pathogens causing huge losses to agriculture and forestry, but culturing- combined current and molecular-based detection method identification-too slow for the rapid identification and implementation of countermeasures.
Here we develop new protocols and quick for third-generation diagnostic Minion-based Oxford Nanopore plant pathogens greatly improve diagnostic speed. However, due to a high error rate and the technical bias in Minion, Pacific BioSciences Sequel platform more useful for deep amplicon based biodiversity monitoring (metabarcoding) from complex environmental samples.
Relative Performance of MinION (Oxford Nanopore Technologies) vs. Sequel (Pacific Biosciences) Third-Generation Sequencing Instruments in Identification of Agricultural and Forest Fungal Pathogens.
Integrative Biology and Big-Data-centrism: Mapping a Bioscience Ethics Perspective with S.W.O.T. Matrix.
In biomedicine today, omics technology-oriented system encourages research mode to achieve a more holistic view and personal health and disease. This scientific approach shifts co-occur with biocapitalism era marked by the market for biomaterials (eg, DNA, cells and tissues) as resource exploitation, high-throughput technology as a tool, and “Big Data” as currency.
Prediagnostics and based genomic analysis successfully entered the public domain more or less unfiltered, offers a variety of business opportunities imagine individuals to contribute to the health sector by providing biomaterials and data, and using technology, thus becoming participants and coproducers health information. Explore the strengths and weaknesses, opportunities and threats by S.W.O.T. analysis, we highlight some of the opportunities, pitfalls, and the sector bias of ethical attitudes Bioscience.
Explore the strengths and weaknesses, opportunities and threats by S.W.O.T. analysis, we highlight some of the opportunities, pitfalls, and the sector bias of ethical attitudes Bioscience. We conclude that the shift from predictive diagnostic interpretation of data that comes along with integrative biology seems to escape occasional awareness of the public and experts.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Histone Deacetylase 2 (HDAC2) in samples from tissue homogenates, cell lysates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Histone Deacetylase 2 (HDAC2) in samples from tissue homogenates, cell lysates or other biological fluids.
Description: The histone deacetylase (HDAC) family contains multiple members which are divided into four classes. Class I of the HDAC family comprises four members, HDAC1, 2, 3, and 8, each of which contains a deacetylase domain and exhibits a different, individual substrate specificity and function in vivo (1). HDAC2 was first identified as as a mammalian homolog to the yeast transcriptional regulator RPD3 (2). HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor (2,3). Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events (4).
Description: The histone deacetylase (HDAC) family contains multiple members which are divided into four classes. Class I of the HDAC family comprises four members, HDAC1, 2, 3, and 8, each of which contains a deacetylase domain and exhibits a different, individual substrate specificity and function in vivo (1). HDAC2 was first identified as as a mammalian homolog to the yeast transcriptional regulator RPD3 (2). HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor (2,3). Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events (4).
Description: A polyclonal antibody against HDAC2. Recognizes HDAC2 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:5000, IHC:1:25-1:100
Description: A polyclonal antibody against HDAC2. Recognizes HDAC2 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:2000, IHC:1:15-1:50
Description: A polyclonal antibody against HDAC2. Recognizes HDAC2 from Human, Mouse, Rat, Monkey. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/40000
Description: A polyclonal antibody against HDAC2. Recognizes HDAC2 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF
Description: Histone deacetylase 2 is an enzyme that in humans is encoded by the HDAC2 gene. This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues on the N-terminal region of the core histones(H2A, H2B, H3 and H4). This protein also forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Betz et al.(1998) performed PCR using HDAC2-specific primers to screen a somatic cell hybrid mapping panel. They mapped the HDAC2 gene to human chromosome 6q21, a region of the genome altered in some cancers, including retinoblastoma.
Description: A polyclonal antibody for detection of HDAC2 from Human, Mouse, Rat, Monkey. This HDAC2 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human HDAC2 around the non-phosphorylation site of S394
Description: A polyclonal antibody for detection of HDAC2 from Human, Mouse, Rat, Monkey. This HDAC2 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human HDAC2 around the non-phosphorylation site of S394
Description: A polyclonal antibody for detection of HDAC2 from Human, Mouse, Rat, Monkey. This HDAC2 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human HDAC2 around the non-phosphorylation site of S394
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human HDAC2 . This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human HDAC2 . This antibody is tested and proven to work in the following applications:
Description: A Rabbit Polyclonal antibody against HDAC2 from Human/Mouse/Rat/Monkey. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA
Description: A Rabbit Polyclonal antibody against HDAC2 from Human/Mouse/Rat/Monkey. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA
Description: This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants.
Description: This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants.
Description: This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants.
Description: Recombinant human IP-10 (also known as CXCL10) is a disulfide-linked homodimeric protein consisting of 78 amino acid residues, and migrates as an approximately 9 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human IP-10 mature chain was expressed in E. coli.
Description: Recombinant human IP-10 (also known as CXCL10) is a disulfide-linked homodimeric protein consisting of 78 amino acid residues, and migrates as an approximately 9 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human IP-10 mature chain was expressed in E. coli.
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human HDAC2 (S394). This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human HDAC2 (Center). This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human HDAC2 (Center). This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody against Phospho-HDAC2 (S394). Recognizes Phospho-HDAC2 (S394) from Human, Mouse, Rat, Monkey. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/20000
Description: Mouse Monoclonal HDAC2 Antibody (Monoclonal). Validated in ChIP, ChIP-qPCR, ChIP-seq, IF and tested in Human.
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In addition, the rapid translation into products for the global healthcare market is based on the valuable views on health and disease, which in turn affects the basic research through, for example, funding policy and research questions that are asked.
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